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Human Mig chemokine: biochemical and functional characterization
F Liao1, R L Rabin, J R Yannelli
1Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
The Journal of Experimental Medicine
|November 1, 1995
Summary
Mig, a chemokine, is induced by interferon-gamma and attracts T cells. Recombinant Mig (rHuMig) shows activity in T lymphocytes but not other immune cells, with variations in its structure affecting function.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- Mig is a CXC chemokine inducible by interferon-gamma (IFN-γ) in macrophages.
- Chemokines play crucial roles in immune cell trafficking and activation.
Purpose of the Study:
- To characterize the biological activity and processing of human Mig (HuMig).
- To investigate the role of HuMig in immune cell responses.
Main Methods:
- Transfection of Chinese hamster ovary (CHO) cells with HuMig cDNA for recombinant protein production.
- Calcium flux assays and modified Boyden chamber assays to assess immune cell responses.
- SDS-PAGE to analyze secreted HuMig species heterogeneity.
Main Results:
- Recombinant human Mig (rHuMig) induced calcium flux and chemotaxis in human tumor-infiltrating T lymphocytes (TIL) and activated peripheral blood lymphocytes.
- rHuMig did not elicit responses in neutrophils, monocytes, or B lymphoblastoid cell lines.
- Secreted HuMig exhibited heterogeneity due to carboxy-terminal proteolytic cleavage, with truncated forms showing reduced activity.
Conclusions:
- HuMig is a specific chemoattractant for T lymphocytes.
- Proteolytic processing of HuMig affects its biological activity.
- HuMig likely plays a significant role in T cell trafficking and T cell-mediated immunity.