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Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
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Published on: June 3, 2012

The matrix protein gene determines amantadine-sensitivity of influenza viruses

A J Hay, N C Kennedy, J J Skehel

    The Journal of General Virology
    |January 1, 1979
    PubMed

    Insights

    Influenza virus resistance to amantadine is controlled by a single gene. This gene codes for the matrix protein, determining the transfer of amantadine resistance or sensitivity in recombinant strains.

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Influenza viruses are a significant global health concern.
    • Amantadine is an antiviral drug historically used against influenza.
    • Understanding the genetic basis of antiviral resistance is crucial for developing effective treatments.

    Purpose of the Study:

    • To investigate the genetic basis of amantadine resistance in influenza virus.
    • To identify the specific gene responsible for amantadine sensitivity or resistance.

    Main Methods:

    • Generation of recombinant influenza virus strains through mixed infections.
    • Comparison of the genetic makeup of these recombinant strains.
    • Assessment of the antiviral response of the strains to amantadine.

    Main Results:

    • Recombinant strains exhibited varying responses to amantadine.
    • A single gene was identified as the determinant of amantadine resistance or sensitivity.
    • This gene encodes the influenza virus matrix protein.

    Conclusions:

    • The matrix protein gene is the sole determinant of amantadine resistance in influenza virus.
    • This finding has implications for the development of new antiviral strategies.
    • Further research can focus on targeting the matrix protein for therapeutic interventions.

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