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A Tn4001 delivery system for Streptococcus gordonii (Challis)
1Laboratory of Microbial Ecology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892, USA.
Plasmid
|March 1, 1995
Summary
The Staphylococcus aureus transposon Tn4001 can successfully transfer within Streptococcus gordonii, creating genetic mutations. This transposon offers a stable and versatile tool for streptococcal genetic research.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Staphylococcus aureus transposon Tn4001 is a mobile genetic element.
- Streptococcus gordonii is a common oral bacterium.
- Genetic tools for streptococci are valuable for research.
Purpose of the Study:
- To investigate the transposition of Staphylococcus aureus transposon Tn4001 in Streptococcus gordonii.
- To evaluate the utility of Tn4001 as a genetic tool in streptococci.
Main Methods:
- Introducing the Tn4001 transposon into Streptococcus gordonii.
- Analyzing transposition sites and element stability.
- Generating lactose-negative mutants to demonstrate utility.
Main Results:
- Tn4001 demonstrated transposition in Streptococcus gordonii with random site distribution.
- The transposon was stable without antibiotic selection.
- Transposition frequency increased when using a Dam- Dcm- Escherichia coli host.
- Tn4001 successfully generated lactose-negative mutants.
Conclusions:
- Tn4001 is a functional and stable transposon in Streptococcus gordonii.
- Its characteristics make it a valuable tool for streptococcal genetics.
- Further applications in generating specific mutants are possible.