[The application of halo assay in analysis of cell chromatin structure]

Halo assay, a method for quantitative analysis of chromatin structure in cell, was developed recently. Due to visualization, feasibility, rapidity, and sensitivity of the method, it has been applied in radiobiology and cancer research. According to previous reports, with some improvement, this method has been established in our laboratory. Employing halo assay, the chromatin structure of two kinds of cell strain with similar genetic background and different radiosensitivity was analyzed and the results show that radiosensitive strain has looser chromatin structure than radioresistant one. It is possible that this difference is related to their different radiosensitivity, thus it's a proof for the relation of chromatin structure and radiosensitivity.