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Updated: Aug 13, 2026
Micropatterning and Assembly of 3D Microvessels
Published on: September 9, 2016
[Culture and identification of human vascular endothelial cells]
1Zhongshan Ophthalmic Center, Sun Yet-sen University of Medical Sciences, Guangzhou, 510060.
Purpose:
To investigate the culture technigue of human vascular endothelial cells in vitro and improve the success rate of culture.
Methods:
Human vascular endothelial cells were isolated from umbilical cords by "Irrigative digestion" technique with 0.2% collagenase III and were digested by 0.0625% tripsin when the cells confluent. The cells were identified by morphologic and immunohistochemical assays.
Results:
The cultured human umbilical vein endothelial cells attached completely after 24 hours in vitro, reached confluence in 3-5 days with the typical appearance of "stone of pave road" and were passaged on time; Immunohistochemically these cells were stained positively by CD34 monoclonal antibody.
Conclusion:
"Irrigative digestion" technique was a reliable method to obtain human umbilical vascular endothelial cells with high success rate. It can be widely used to the culture and researches of human vascular endothelial cells.
