Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Design and implementation of a database and program for 3D reconstruction from serial sections: a data-driven

F J Verbeek1, D P Huijsmans, R J Baeten

  • 1Department of Anatomy and Embryology, Faculty of Medicine, University of Amsterdam, The Netherlands.

Microscopy Research and Technique
|April 15, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of co-localization of objects in dual-colour confocal images.

Journal of microscopy·2021
Same author

Is hepatocellular carcinoma the same disease in children and adults? Comparison of histology, molecular background, and treatment in pediatric and adult patients.

Pediatric blood & cancer·2018
Same author

Hypoxia impairs adaptation of skeletal muscle protein turnover- and AMPK signaling during fasting-induced muscle atrophy.

PloS one·2018
Same author

Medical students' perspective on training in anatomy.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft·2018
Same author

Unexpected regulation of miRNA abundance during adaptation of early-somite mouse embryos to diabetic pregnancy.

Biochemical and biophysical research communications·2016
Same author

Cushing syndrome as presenting symptom of calcifying nested stromal-epithelial tumor of the liver in an adolescent boy: a case report.

Journal of medical case reports·2016

This study presents a streamlined computer program for 3D reconstruction from serial sections, simplifying data handling for developmental biologists. The contour model approach facilitates easy visualization of complex biological structures.

Area of Science:

  • Developmental Biology
  • Biotechnology
  • Computer Science

Background:

  • Three-dimensional (3D) reconstruction is crucial for understanding complex developmental patterns.
  • Modern immunohistochemical techniques necessitate advanced tools for 3D analysis.
  • Standard laboratory setups require streamlined 3D reconstruction methods.

Purpose of the Study:

  • To describe a structural approach for a standard computer program for 3D reconstruction from serial sections.
  • To simplify data input and manipulation for easier results in 3D reconstruction.
  • To provide a practical tool for developmental biologists to analyze 3D structures.

Main Methods:

  • A combination of strict data ordering and process ordering (information analysis) was employed.

Related Experiment Videos

  • The contour model was chosen as the primary method for building the reconstruction model.
  • Data is organized in a database for efficient manipulation and output.

    • Main Results:

    • A computer program for 3D reconstruction from serial sections was developed and described.
    • The program streamlines data input, inspection, revision, and reconstruction processes.
    • Demonstrated successful 3D reconstruction using an 11 embryonic days (ED) rat embryo and human embryonic heart development.

    Conclusions:

    • The developed structural approach offers an efficient method for 3D reconstruction in standard laboratory settings.
    • The contour model and database system facilitate easy visualization and analysis of biological development.
    • This program serves as a valuable tool for understanding 3D developmental patterns.