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Related Experiment Videos

The toothpick method in relation to other plaque sampling techniques for evaluating mutans streptococci

K Wennerholm1, B Lindquist, C G Emilson

  • 1Department of Cardiology, Faculty of Odontology, Göteborg University, Sweden.

European Journal of Oral Sciences
|February 1, 1995
PubMed
Summary
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Toothpicks effectively detect mutans streptococci on tooth surfaces, offering comparable or superior results to traditional methods like dental floss or needles. This study highlights toothpicks as a reliable tool for assessing bacterial prevalence.

Area of Science:

  • Microbiology
  • Dental Public Health
  • Oral Hygiene

Background:

  • Mutans streptococci are key pathogens in dental caries.
  • Accurate detection methods are crucial for targeted interventions.
  • Comparing sampling techniques is essential for optimizing diagnostic accuracy.

Purpose of the Study:

  • To evaluate the efficacy of toothpicks for detecting mutans streptococci.
  • To compare toothpick sampling with conventional methods (carver, needle, floss).
  • To assess prevalence and colonization levels on various tooth surfaces.

Main Methods:

  • Plaque samples collected from buccal, lingual, occlusal, and approximal surfaces using toothpicks and control methods.
  • Samples cultured on MSB agar; mutans streptococci presence scored (0-4).

Related Experiment Videos

  • Total viable counts determined on blood agar for plaque mass assessment.

    • Main Results:

    • Toothpicks yielded higher total viable counts for buccal/lingual plaque compared to carvers.
    • More tooth surfaces tested positive for mutans streptococci with toothpicks than other methods.
    • Higher colonization scores observed with toothpicks versus carvers or needles; floss showed similar levels to toothpicks.

    Conclusions:

    • Toothpick sampling demonstrates favorable comparison to carvers, needles, and floss for mutans streptococci detection.
    • Toothpicks offer a viable and effective method for assessing bacterial prevalence on individual tooth surfaces.
    • This method aids in understanding and managing cariogenic bacterial populations.