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Related Experiment Videos

Processing of the hepatitis C virus precursor protein

K Shimotohno1, Y Tanji, Y Hirowatari

  • 1Virology Division, National Cancer Center Research Institute, Tokyo, Japan.

Journal of Hepatology
|January 1, 1995
PubMed
Summary

Hepatitis C virus (HCV) polyprotein processing involves at least three pathways, generating over 10 viral proteins. These proteins associate, with some binding to membranes, crucial for viral replication.

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Area of Science:

  • Virology
  • Molecular Biology
  • Biochemistry

Background:

  • Hepatitis C virus (HCV) proteins originate from a single polyprotein precursor.
  • Post-translational processing is essential for generating functional viral proteins.

Purpose of the Study:

  • To analyze the production pathways of HCV proteins.
  • To identify the processing mechanisms and resulting viral proteins.

Main Methods:

  • In vitro translation assays.
  • Plasmid-based transient gene expression in mammalian cell lines.

Main Results:

  • At least three distinct processing pathways were identified, yielding over 10 HCV proteins.
  • Signal peptidase processed structural proteins, producing two E2 variants (gp70 type A and B).

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  • Two viral proteinases processed nonstructural proteins, with distinct functional requirements and overlapping activity in NS3.

    • Conclusions:

    • Multiple processing pathways contribute to the generation of the HCV proteome.
    • Processed HCV proteins form complexes, with some exhibiting membrane association.