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Related Experiment Videos

A giant nucleopore protein that binds Ran/TC4

N Yokoyama1, N Hayashi, T Seki

  • 1Department of Molecular Biology, Graduate School of Medical Science, Kyushu-University, Fukuoka, Japan.

Nature
|July 13, 1995
PubMed
Summary
This summary is machine-generated.

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Researchers identified RanBP2, a novel giant protein that binds to the Ran/TC4 G protein. RanBP2 is a nuclear pore complex component involved in nuclear protein import.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Interactions

Background:

  • Ran/TC4 is a small nuclear G protein crucial for cell cycle progression, RNA export, and nuclear protein import.
  • RCC1 is a guanine nucleotide release factor that forms a complex with Ran/TC4.
  • Ran/TC4 functions downstream of RCC1, as suggested by its ability to suppress RCC1 loss-of-function defects.

Purpose of the Study:

  • To identify novel proteins that bind to Ran/TC4.
  • To characterize the structure and function of newly identified Ran/TC4-binding proteins.

Main Methods:

  • Yeast two-hybrid screening to identify Ran/TC4-interacting proteins.
  • Protein domain analysis and sequence homology searches.
  • Immunolocalization studies to determine protein localization within the cell.

Related Experiment Videos

  • Functional assays using antibodies to assess the role in nuclear import.

    • Main Results:

    • Identification of RanBP2, a novel protein of 3,224 residues, as a Ran/TC4-binding partner.
    • RanBP2 possesses a unique domain structure including leucine-rich repeats, RanBP1-homologous domains, zinc-finger motifs, and cyclophilin homology.
    • RanBP2 contains the XFXFG motif characteristic of nuclear pore complex (NPC) proteins and localizes to the NPC.
    • Antibodies against RanBP2 inhibit NLS-mediated nuclear import, suggesting a role in this process.

    Conclusions:

    • RanBP2 is a novel, large NPC protein that interacts with Ran/TC4.
    • RanBP2 plays a functional role in NLS-mediated nuclear protein import through the NPC.