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Identification of denatured double-stranded DNA by matrix-assisted laser desorption/ionization time-of-flight mass
1Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
Rapid Communications in Mass Spectrometry : RCM
|January 1, 1995
Abstract:
We created double-stranded DNA (dsDNA) by annealing synthetic oligonucleotides and used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to measure the difference in molecular weight of the complementary strands after denaturation. Refined sample preparation and deliberate rectilinear construction at a linear mass spectrometer produced a mass resolution exceeding 1000 for single-stranded DNA. We present methodologies and demonstrate the potential for analysing dsDNA by determining the mass of the complementary strands.