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Updated: Aug 19, 2026
Intracellular Refolding Assay
Published on: January 24, 2012
Heat shock protein 70 and HLA-DR molecules tissue expression. Prognostic implications in colorectal cancer
A C Lazaris1, G E Theodoropoulos, P S Davaris
1Department of Pathology, Medical School of Athens University, Hippokration General Hospital, Greece.
Purpose:
The expression of 70,000-Da heat shock protein (HSP 70) and HLA-DR molecules on cancer cells influences immunologic mechanisms that may be of some prognostic significance. The purpose of this study was to examine the relationship among immunohistochemical HSP 70, HLA-DR expression, and clinicopathologic tumor variables, as well as patient survival in a series of 128 colorectal carcinomas.
Method:
A three-step immunoperoxidase staining technique was undertaken for detection of both markers.
Results:
Of the examined carcinomas 77.3 percent were HSP 70-positive and 74.2 percent were HLA-DR-positive. Increased HSP 70-positive expression correlated significantly with low differentiation (P < 0.05), showed a tendency to characterize advanced stages of disease, and was clearly associated with worse overall survival (P < 0.05). The highest rate of HLA-DR positivity was demonstrated in early stages and was significantly associated with more favorable prognosis (P < 0.001). HSP 70-positive/HLA-DR-negative patients had worse overall survival compared with the rest (P < 0.001).
Conclusions:
The resulting opposite effects on prognosis of examined markers seem to be related to different pathophysiologic functional roles on tumor immunology.
