The murine erythrocyte protein-4.2-encoding gene: similarities and differences in structure and expression from its...

We have isolated a complete cDNA encoding for the mouse erythrocyte protein 4.2 (P4.2). The entire P4.2 cDNA consists of 3465 nt with an open reading frame (ORF) of 691 amino acids. Northern blot analysis of mouse reticulocyte or spleen RNA using the P4.2 cDNA as a probe, detected a 3.5-kb message. The size of the mouse P4.2 cDNA or message that we obtained, appears to be different from those reported recently. Despite the similarity to the human P4.2 cDNAs, the mouse cDNA has a longer 3' untranslated region. A genomic clone covering the first exon and flanking sequences of the mouse P4.2 gene was isolated. Sequencing results from the first exon-intron junction region and polymerase chain reaction (PCR) experiments revealed that the mouse reticulocyte P4.2 RNA does not exhibit alternative splicing in the region identified in the human P4.2 RNA.