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Monoclonal antibody induced hearing loss
T S Nair1, Y Raphael, D F Dolan
1Cell Biology Laboratory, Kresge Hearing Research Institute, University of Michigan, Ann Arbor 48109-0506, USA.
Hearing Research
|March 1, 1995
Summary
Monoclonal antibody KHRI-3 causes hearing loss in mice by damaging the organ of Corti and outer hair cells. This research offers insights into antibody-mediated hearing loss and an animal model for further study.
Area of Science:
- Immunology
- Otolaryngology
- Cell Biology
Background:
- Monoclonal antibodies KHRI-3 and KHRI-5 target inner ear cells.
- The impact of these antibodies on inner ear function requires investigation.
Purpose of the Study:
- To determine if monoclonal antibodies KHRI-3 and KHRI-5 affect inner ear function.
- To investigate the mechanisms underlying antibody-mediated hearing loss.
Main Methods:
- Syngeneic Balb/c mice were inoculated with hybridomas secreting KHRI-3, KHRI-5, or control antibodies.
- Auditory brainstem responses (ABR) were measured before and after inoculation.
- Histological analysis of cochlear tissues was performed.
Main Results:
- KHRI-3 antibody administration led to high-frequency hearing loss (40-50 dB shifts).
- Hearing loss correlated with hybridoma growth, KHRI-3 antibody presence, and increased immunoglobulin titers.
- Histopathology revealed damage to the organ of Corti, specifically outer hair cell loss, in KHRI-3 treated mice.
Conclusions:
- The KHRI-3 antibody induces hearing loss secondary to damage of the organ of Corti and outer hair cells.
- These findings highlight antibody-mediated mechanisms in hearing loss.
- An animal model is established for studying antibody-induced hearing dysfunction.