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An ascending non-obstructive model for chronic pyelonephritis in BALB/c mice
R Gupta1, N K Ganguly, V Ahuja
1Department of Microbiology, Government Medical College, Chandigarh, India.
Journal of Medical Microbiology
|July 1, 1995
Summary
This study successfully modeled chronic pyelonephritis in mice using Escherichia coli. A non-pilate E. coli strain induced greater inflammation and renal scarring than a pilate strain.
Area of Science:
- Microbiology
- Immunology
- Nephrology
Background:
- Chronic pyelonephritis is a serious kidney infection.
- Understanding the role of bacterial virulence factors like pili is crucial for developing effective treatments.
Purpose of the Study:
- To establish a mouse model for chronic pyelonephritis using Escherichia coli.
- To compare the pathogenicity of an E. coli strain expressing type-1 pili versus a non-pilate mutant.
Main Methods:
- Female BALB/c mice were infected with two strains of Escherichia coli via the ascending route.
- Strain 31-B expressed type-1 pili, while strain BH-5 was a non-pilate mutant.
- Renal inflammation and scarring were assessed 5 months post-infection.
Main Results:
- Both E. coli strains induced chronic renal inflammation.
- The non-pilate strain (BH-5) caused a significantly greater inflammatory response.
- The non-pilate strain also led to more pronounced renal scarring compared to the pilate strain (31-B).
Conclusions:
- Type-1 pili expression in Escherichia coli may attenuate the inflammatory response and reduce renal scarring in a chronic pyelonephritis model.
- Bacterial factors beyond initial colonization, such as pili expression, significantly influence disease severity and long-term kidney damage.