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Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
Published on: September 3, 2014
Involvement of HPC-1/syntaxin-1A antigen in transmitter release from PC12h cells
Y Kushima1, T Fujiwara, T Morimoto
12nd Department of Physiology, Kyorin University, School of Medicine, Tokyo, Japan.
Abstract:
We examined the effect of antiserum against HPC-1/Syntaxin-1A on the norepinephrine release from digitonin-permeabilized PC12h cells. PC12h cells were permeabilized with digitonin and preincubated with nonimmunized serum or antiserum against HPC-1. The release of norepinephrine was measured in the presence or absence of calcium. The calcium-dependent norepinephrine release was increased in the cells preincubated with anti HPC-1 antiserum. However, with a higher concentration of anti HPC-1 antiserum, the calcium-dependent norepinephrine release was decreased, possibly because of a nonspecific effect. In the case of purified IgG, the same results were obtained. These findings suggested that HPC-1 plays an important role in the exocytosis of transmitter presumably by suppressing the membrane fusion process between the synaptic vesicle and presynaptic membrane.
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