Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sample preparation for Chlamydia pneumoniae PCR

J Gnarpe1, K Eriksson

  • 1Department of Clinical Microbiology, Gävle Hospital, Sweden.

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
|April 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epidemiology of childhood neuromuscular diseases in two hospital districts in Finland.

European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society·2025
Same author

The value of CSF diagnostic and prognostic biomarkers in NMOSD and MOGAD in real-life use.

Multiple sclerosis and related disorders·2025
Same author

The state of the art in beyond 5G distributed massive multiple-input multiple-output communication system solutions.

Open research Europe·2023
Same author

Intellectual disability in children aged less than seven years born moderately and late preterm compared with very preterm and term-born children - a nationwide birth cohort study.

Journal of intellectual disability research : JIDR·2017
Same author

Lifestyle habits and fatigue among people with systemic lupus erythematosus and matched population controls.

Lupus·2015
Same author

Physical activity in patients with systemic lupus erythematosus and matched controls.

Scandinavian journal of rheumatology·2012
Same journal

Immune Cell Composition and Prognosis in Node-Positive, Irradiated Breast Cancer Patients in the DBCG-IMN2 Study.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same journal

Urinary Secretory-IgA and IgG Responses and Inflammatory Markers in Patients With Pseudomonas aeruginosa Urinary Tract Infections.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same journal

Predictive Immunohistochemistry in Cholangiocarcinoma: Current Clinical Utility, Practical Limitations, and Emerging Directions.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same journal

Active Chlorine Antiseptics in Wound Care: Recent Advances and Perspectives for Military Medicine.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same journal

A Review on the Logistics, Financial, Ethical, and Regulatory Frameworks of Artificial Intelligence in Digital Pathology.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same journal

UE, CT, and ACR TI-RADS Grading in the Combined Diagnosis of Benign and Malignant Thyroid Nodules.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
See all related articles

This study compared two sample preparation methods for detecting Chlamydia pneumoniae via PCR in respiratory infections. The Amplicor kit significantly improved detection rates compared to proteinase K treatment.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Respiratory tract infections (RTIs) are common, with Chlamydia pneumoniae being a significant pathogen.
  • Accurate and sensitive detection of C. pneumoniae is crucial for effective diagnosis and treatment of RTIs.
  • Current diagnostic methods, including polymerase chain reaction (PCR), can be influenced by sample preparation techniques.

Purpose of the Study:

  • To evaluate and compare the efficacy of two distinct sample preparation methods for the detection of Chlamydia pneumoniae in clinical specimens from patients with chronic respiratory infections.
  • To determine if enhanced cell lysis during sample preparation improves PCR-based detection rates of C. pneumoniae.

Main Methods:

  • Fifty-three retropharyngeal mucosal specimens from patients with long-standing RTIs were analyzed.

Related Experiment Videos

  • Specimens were divided into two aliquots for parallel testing.
  • One aliquot was treated with proteinase K, while the other underwent preparation using the Amplicor sputum sample preparation kit.
  • Both sample types were subjected to PCR using a primer pair specific for Chlamydia pneumoniae.

    • Main Results:

    • The Amplicor kit preparation method yielded a positivity rate of 20.8% for C. pneumoniae.
    • The proteinase K method resulted in a lower positivity rate of 7.5%.
    • Improved C. pneumoniae detection correlated with sample preparation methods ensuring more complete cell lysis.

    Conclusions:

    • The Amplicor sputum sample preparation kit is a more effective method than proteinase K for detecting Chlamydia pneumoniae in retropharyngeal mucosal specimens.
    • Optimizing sample preparation, particularly achieving thorough cell lysis, is critical for enhancing the sensitivity of PCR-based diagnostics for C. pneumoniae infections.