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Enzymic assay for lecithin in amniotic fluid

S Anaokar, P J Garry, J C Standefer

Clinical Chemistry

|January 1, 1979

Summary

A new enzymic method accurately measures lecithin in amniotic fluid. This approach provides a faster, more reliable alternative for assessing fetal lung maturity compared to traditional lecithin/sphingomyelin ratio tests.

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Area of Science:

Biochemistry
Clinical Chemistry
Enzymology

Background:

Lecithin determination in amniotic fluid is crucial for assessing fetal lung maturity.
Existing methods, such as the lecithin/sphingomyelin ratio, can be time-consuming and require larger sample volumes.
A need exists for a more efficient and accurate diagnostic tool.

Purpose of the Study:

To develop and validate a novel enzymic method for quantifying lecithin in amniotic fluid.
To establish a reliable alternative for fetal lung maturity assessment.

Main Methods:

Utilized Phospholipase D from savoy cabbage to hydrolyze lecithin, releasing choline.
Employed choline oxidase from Arthrobacter globiformis to oxidize choline, producing hydrogen peroxide.
Quantified hydrogen peroxide via a spectrophotometric assay involving a colored complex formation with phenol and 4-aminoantipyrene in the presence of horseradish peroxidase.

Main Results:

The described enzymic method demonstrated ease of use, precision, and accuracy.
The assay requires less time and a smaller sample volume compared to existing techniques.
The method proved to be a dependable alternative for evaluating fetal lung maturity.

Conclusions:

The novel enzymic assay offers a reliable, efficient, and accurate method for determining amniotic fluid lecithin.
This technique serves as a valuable tool for assessing fetal lung maturity, potentially replacing traditional ratio-based methods.

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