Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Local pressure effects on vitreous kinetics

Y Tsukahara1, D M Maurice

  • 1Department of Ophthalmology, Stanford University School of Medicine, CA, USA.

Experimental Eye Research
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell death signalling is competitively but coordinately regulated by repressor-type and activator-type ethylene response factors in tobacco (Nicotiana tabacum) plants.

Plant biology (Stuttgart, Germany)·2022
Same author

Autoimmune pulmonary alveolar proteinosis developed during immunosuppressive treatment in polymyositis with interstitial lung disease: a case report.

BMC pulmonary medicine·2020
Same author

Across and within breed differences in the relationship between packed cell volume and fecal egg count in growing meat goat and hair sheep males naturally and artificially infected with gastrointestinal nematodes.

Veterinary parasitology, regional studies and reports·2019
Same author

Effect of Ovar-DRA and Ovar-DRB1 genotype in small ruminants with haemonchosis.

Parasite immunology·2018
Same author

EVALUATION OF PATIENT EXPOSURE IN FAST kVp SWITCHING DUAL ENERGY COMPUTED TOMOGRAPHY: PHANTOM STUDY.

Radiation protection dosimetry·2018
Same author

Effects of level of brackish water on feed intake, digestion, heat energy, and blood constituents of growing Boer and Spanish goat wethers.

Journal of animal science·2016

Subconjunctival injection of fluorescein (F) and carboxyfluorescein (CF) dyes into rabbit eyes showed that local pressure significantly increased dye penetration into the vitreous. Mathematical modeling helped analyze dye kinetics and retinal permeability.

Area of Science:

  • Ophthalmology
  • Pharmacokinetics
  • Biomedical Engineering

Background:

  • Understanding drug delivery to the posterior segment of the eye is crucial for treating ocular diseases.
  • Fluorescein and carboxyfluorescein are commonly used tracers for ocular penetration studies.
  • Ocular drug delivery is often limited by biological barriers and transport mechanisms.

Purpose of the Study:

  • To investigate the effect of local pressure on the penetration and elimination kinetics of fluorescein and carboxyfluorescein in the rabbit eye.
  • To compare experimental findings with predictions from a mathematical diffusion model.
  • To identify physiological parameters influencing dye transport across the retina and into the vitreous.

Main Methods:

  • Subconjunctival injection of fluorescein (F) or carboxyfluorescein (CF) in rabbit eyes.

Related Experiment Videos

  • Application of local pressure over the injection site to occlude choroidal circulation.
  • Measurement of dye penetration into and loss from the vitreous body and anterior segment.
  • Transcleral iontophoresis of fluorescein as an alternative delivery method.
  • Comparison of experimental data with a mathematical diffusion model.

    • Main Results:

    • Local pressure increased fluorescein penetration ~30-fold and carboxyfluorescein penetration ~7-fold, while delaying fluorescein loss.
    • Cooling the tissue with local pressure had minimal additional effect on dye penetration.
    • Transcleral iontophoresis of fluorescein showed no effect of local pressure on dye kinetics.
    • The mathematical model accurately predicted dye kinetics in some experiments, but discrepancies suggested additional mechanisms.

    Conclusions:

    • Local pressure significantly enhances dye penetration into the vitreous, likely by altering local blood flow and tissue permeability.
    • While a diffusion model explains some aspects of dye transport, factors like retinal storage and anterior pathway penetration are also important.
    • These findings have implications for optimizing drug delivery strategies to the posterior eye segment.