Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endogenous antigen presentation by MHC class II molecules

A J Sant1

  • 1Department of Pathology, University of Chicago, IL 60637, USA.

Immunologic Research
|January 1, 1994
PubMed
Summary

Major histocompatibility complex (MHC) class II molecules present endogenous antigens synthesized within antigen-presenting cells (APCs). Most peptides bound by MHC class II originate from intracellular proteins, with ongoing research into antigen presentation mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A funnel approach to enable analyses of epitope-specific human CD4 T cells specific for influenza and SARS-CoV-2.

mBio·2026
Same author

Light transmission from a randomly rough dielectric diffuser: theoretical and experimental results.

Optics letters·2009
Same author

Comparison of surface scattering between identical, randomly rough metal and dielectric diffusers.

Optics letters·2009
Same author

Hydrogen bond integrity between MHC class II molecules and bound peptide determines the intracellular fate of MHC class II molecules.

Journal of immunology (Baltimore, Md. : 1950)·2001
Same author

Energetic asymmetry among hydrogen bonds in MHC class II*peptide complexes.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

DM determines the cryptic and immunodominant fate of T cell epitopes.

The Journal of experimental medicine·2000

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • T cell recognition of antigens involves complexes of peptides and major histocompatibility complex (MHC) molecules.
  • MHC class II molecules present both exogenous and endogenous antigens to CD4 T cells.

Purpose of the Study:

  • This review focuses on the mechanisms of endogenous antigen presentation by MHC class II molecules.
  • To explore the intracellular pathways and sites of peptide loading onto MHC class II.

Main Methods:

  • Isolation and amino acid sequencing of peptides bound to MHC class II molecules.
  • Studies using model antigens to investigate intracellular trafficking and presentation.

Main Results:

  • 70-90% of peptides presented by MHC class II are derived from intracellularly synthesized proteins.
  • Proteins from various intracellular compartments (cell surface, ER, cytosol) can access MHC class II, with varying efficiencies.

Conclusions:

  • Key questions remain regarding intracellular trafficking, peptide loading sites, and cell-type specific variations in antigen presentation.
  • Variability in presented peptides could influence self-tolerance and autoimmunity.

Related Experiment Videos