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[Delta-Aminolevulinic acid in urine (screening method)]
1Department of Community Health Science, Saga Medical School.
Nihon Rinsho. Japanese Journal of Clinical Medicine
|June 1, 1995
Summary
This review details methods for measuring urinary delta-aminolevulinic acid (ALA). It compares colorimetric and fluorometric High-Performance Liquid Chromatography (HPLC) techniques for ALA determination.
Area of Science:
- Biochemistry
- Analytical Chemistry
Context:
- Urinary delta-aminolevulinic acid (ALA) determination is crucial for diagnosing certain metabolic disorders.
- Numerous analytical methods have been developed since 1956.
Purpose:
- To provide a historical overview of urinary ALA determination methodologies.
- To compare the advantages and disadvantages of different analytical approaches.
Summary:
- Methods for urinary ALA determination are broadly categorized into colorimetric and fluorometric techniques.
- Colorimetric methods are user-friendly and cost-effective but lack specificity.
- Fluorometric methods, particularly those coupled with High-Performance Liquid Chromatography (HPLC), offer high sensitivity and specificity but require expensive instrumentation.
Impact:
- Understanding the evolution of ALA measurement techniques aids in selecting appropriate diagnostic tools.
- Highlights the trade-offs between cost, convenience, specificity, and sensitivity in analytical method selection.