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The release of TNF-alpha (factor) by peritoneal macrophages of hamsters bearing transplantable melanomas
1Department of Histology and Immunology, Medical School, Gdańsk, Poland.
Abstract:
The influence of two kinds of transplantable melanomas on the secretory function of peritoneal macrophages, and particularly the release of TNF-alpha has been studied. The results showed a statistically significant increase of protein content in the supernatants from 24 h cultured macrophages from melanoma-bearing hamsters in comparison with control macrophages. The release of TNF-alpha by control macrophages was higher than that by macrophages of hamsters with transplantable melanomas. The decrease in release of this factor was more prominent in case of macrophages from hamsters bearing amelanotic melanoma, it may suggest that biological features of melanomas can influence peritoneal macrophages to release the TNF-alpha at different level.
Insights
Tumor growth in hamsters significantly increased protein in macrophage cultures. However, macrophages from tumor-bearing hamsters released less tumor necrosis factor-alpha (TNF-alpha), especially with amelanotic melanoma.
Area of Science:
- Immunology
- Oncology
- Cell Biology
Background:
- Peritoneal macrophages play a crucial role in immune responses.
- Tumor microenvironments can modulate macrophage function.
- Tumor necrosis factor-alpha (TNF-alpha) is a key cytokine in inflammation and immunity.
Purpose of the Study:
- To investigate the impact of transplantable melanomas on peritoneal macrophage secretory function.
- To specifically examine the release of TNF-alpha by macrophages in the presence of melanoma.
Main Methods:
- Culturing peritoneal macrophages from melanoma-bearing and control hamsters for 24 hours.
- Measuring total protein content in cell culture supernatants.
- Quantifying the release of TNF-alpha from macrophages.
Main Results:
- A statistically significant increase in total protein content was observed in supernatants from macrophages of melanoma-bearing hamsters compared to controls.
- Macrophages from control hamsters exhibited higher TNF-alpha release than those from hamsters with melanomas.
- A more pronounced decrease in TNF-alpha release was noted in macrophages from hamsters with amelanotic melanoma.
Conclusions:
- Transplantable melanomas alter peritoneal macrophage secretory function.
- Melanoma presence suppresses TNF-alpha release from peritoneal macrophages.
- The biological characteristics of melanomas, such as amelanotic type, may differentially affect TNF-alpha production by macrophages.
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