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Evidence for oxidative stress during in vitro dialysis
J Westhuyzen1, C E Adams, S J Fleming
1Department of Pathology, Royal Brisbane Hospital, Brisbane, Australia.
Nephron
|January 1, 1995
Summary
Hemodialysis causes oxidative stress and lipid peroxidation, indicated by decreased blood thiols and increased malondialdehyde (MDA). This study used an in vitro dialysis model to investigate these effects in hemodialysis patients.
Area of Science:
- Biochemistry
- Nephrology
- Oxidative Stress Research
Background:
- The role of oxidative stress during hemodialysis remains debated.
- Oxidative stress and lipid peroxidation are implicated in various chronic diseases.
Purpose of the Study:
- To investigate markers of oxidative stress and lipid peroxidation during an in vitro hemodialysis circuit.
- To determine if hemodialysis itself induces oxidative damage.
Main Methods:
- An in vitro dialysis circuit using blood from 7 hemodialysis patients was established.
- Blood was circulated through a cuprophane dialyzer (+FIL group) or without a dialyzer (-FIL group) for 4 hours.
- Concentrations of antioxidants and malondialdehyde (MDA) were measured, alongside red blood cell susceptibility to lipid peroxidation.
Main Results:
- Blood thiol concentrations significantly decreased in the presence of the dialyzer (+FIL group), but not without it (-FIL group).
- Plasma malondialdehyde (MDA) concentrations increased in both groups, indicating lipid peroxidation.
- The susceptibility of red blood cells to lipid peroxidation remained unchanged after 120 minutes of dialysis.
Conclusions:
- These in vitro findings support the hypothesis that hemodialysis is associated with measurable oxidative stress.
- The study demonstrates that hemodialysis can lead to plasma lipid peroxidation.