Related Experiment Video
Updated: Jul 30, 2026
06:45
Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
Published on: May 26, 2011
[Isolation and characteristics of the thyroxine membrane receptor]
Voprosy Meditsinskoi Khimii
|November 1, 1994
Summary
Researchers isolated a thyroxin receptor from rat liver plasma membranes. This glycoprotein receptor, with a 40 kDa mass, was successfully incorporated into artificial membranes for further study.
Area of Science:
- Biochemistry
- Molecular Biology
- Cellular Biology
Context:
- Thyroxin receptors play a crucial role in regulating cellular metabolism and gene expression.
- Understanding receptor structure and function is key to developing targeted therapies for thyroid-related disorders.
- Rat liver plasma membranes serve as a common model system for studying hormone receptor interactions.
Purpose:
- To isolate and characterize the thyroxin receptor from rat liver plasma membranes.
- To determine the molecular properties of the purified thyroxin receptor, including its mass and amino acid composition.
- To assess the functional incorporation of the purified receptor into artificial phospholipid membranes.
Summary:
- A thyroxin receptor was successfully isolated from rat liver plasma membranes using affinity chromatography.
- The purified receptor was identified as a glycoprotein with a molecular mass of 40 kDa, comprising 322 amino acid residues.
- The isolated thyroxin receptor was subsequently incorporated into artificial phospholipid membranes, enabling further investigation of its biophysical properties and interactions.
Impact:
- Provides a purified thyroxin receptor for detailed structural and functional analyses.
- Facilitates studies on the mechanism of thyroxin binding and signal transduction.
- Enables the development of in vitro models for drug screening and therapeutic development targeting the thyroxin receptor.
More Related Videos
12:25Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Published on: September 28, 2018
16:02Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
Published on: February 10, 2023
Related Concept Videos
01:08Intracellular Hormone Receptors
Lipid-soluble hormones diffuse across the plasma and nuclear membrane of target cells to bind to their specific intracellular receptors. These receptors act as transcription factors that regulate gene expression and protein synthesis in the target cell
01:31Internal Receptors
Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
01:27Transducer Mechanism: Enzyme-Linked Receptors
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
01:23The Thyroid Gland
The thyroid gland is a small, butterfly-shaped gland located in the neck and covers the anterior surface of the trachea. The gland has two lateral lobes connected by a thin tissue mass called the isthmus. Internally, each lobe comprises many small spherical structures known as thyroid follicles, surrounded by a network of blood vessels.
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
01:20Synthesis and Regulation of Thyroid Hormones
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
01:18Functions of Thyroid Hormones
The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...