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TRAMP: a software package for generating transposon maps

E Veklerov, C H Martin, E H Theil

    Computer Applications in the Biosciences : CABIOS
    |April 1, 1995
    PubMed
    Summary
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    TRAMP is a software package that generates transposon maps for DNA sequencing. Its selection algorithm identifies the most robust map with minimal inserts, aiding directed sequencing projects.

    Area of Science:

    • Bioinformatics
    • Computational Biology
    • Genomics

    Background:

    • DNA sequencing projects require efficient methods for generating transposon maps.
    • Directed sequencing strategies necessitate robust mapping tools for accurate DNA analysis.
    • Existing software may lack comprehensive automation or user-controlled flexibility.

    Purpose of the Study:

    • To introduce TRAMP, a software package designed for automated transposon map generation.
    • To highlight TRAMP's core selection algorithm for optimizing DNA sequence maps.
    • To present TRAMP as a versatile tool for directed DNA sequencing projects.

    Main Methods:

    • TRAMP utilizes automated tools for transposon map generation.
    • A central selection algorithm identifies the most robust map with the fewest inserts.

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  • The software allows for user override of automated functions.

    • Main Results:

    • TRAMP has been successfully used daily since 1994 by the LBL sequencing team.
    • The software generates comprehensive transposon maps for DNA sequencing.
    • It provides an optimized map with a minimal number of inserts.

    Conclusions:

    • TRAMP is a reliable and effective software package for generating transposon maps.
    • The package's automated yet user-controllable features enhance its applicability.
    • TRAMP is suitable for any DNA sequencing project employing a directed strategy.