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Immunoblot study of IgE binding allergens in celery roots
S Vieths1, A Jankiewicz, B Wüthrich
1Institute of Food Chemistry, Technische Universität Berlin, Germany.
Summary
Celery allergens were identified, including a Bet v I-related protein important for birch pollen-sensitized individuals and celery profilin significant in mugwort pollen-sensitized patients. This research clarifies cross-reactivity in pollen-food allergy syndromes.
Area of Science:
- Allergen characterization
- Immunology
- Food allergy research
Background:
- Celery sensitization is common in patients with mugwort and birch pollen allergies due to cross-reactive IgE antibodies.
- Profilin is a known cross-reactive allergen in celery, birch, and mugwort pollen.
- Limited data exists on other celery allergens.
Purpose of the Study:
- Characterize IgE-binding components in celery root using immunoblot analysis.
- Investigate the cross-reactive nature of celery allergens.
Main Methods:
- Investigated sera from 38 patients with sensitization to birch pollen, mugwort pollen, and/or celery.
- Utilized immunoblot and immunoblot inhibition techniques with celery extract and purified Bet v I.
- Affinity isolated and analyzed celery profilin via immunoblotting.
Main Results:
- A 16-kDa celery allergen cross-reacting with Bet v I was recognized by 6/8 patients with birch pollen/celery sensitization.
- Celery profilin (15-16 kDa) was identified in 6/16 patients with mugwort pollen/celery or triple sensitization.
- Three sera showed IgE antibodies against carbohydrate determinants.
Conclusions:
- A 16-kDa Bet v I-related celery protein is a key allergen for individuals sensitized to birch pollen and celery.
- IgE binding to celery profilin is more significant in patients with additional mugwort pollen sensitization.