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The human gene encoding the largest subunit of RNA polymerase II
1Division of Biology, National Institute of Radiological Sciences, Chiba, Japan.
Gene
|July 4, 1995
Summary
Researchers determined the gene structure of human RNA polymerase II large subunit (RpII LS). The 5' flanking region shows conservation with mouse RpIILS and contains regulatory elements suggesting transcriptional control.
Area of Science:
- Molecular Biology
- Genetics
- Gene Regulation
Background:
- RNA polymerase II (RpII) is crucial for gene transcription in eukaryotes.
- Understanding the structure and regulatory elements of RpII genes is vital for deciphering gene expression control.
Purpose of the Study:
- To determine the complete gene structure of the human RNA polymerase II large subunit (RpII LS).
- To analyze the 5' flanking region for potential regulatory sequences and conserved elements.
Main Methods:
- Nucleotide sequencing of the human RpII LS gene.
- Bioinformatic analysis of the 5' flanking region for conserved sequences and transcription factor binding sites.
Main Results:
- The human RpII LS gene comprises 29 exons.
- The 5' flanking region is highly conserved with the mouse homolog.
- Identified SP1-binding sites, CCAAT sequences, heat-shock element homology, inverted repeats, and palindrome sequences in the 5' region.
Conclusions:
- The 5' flanking region of the human RpII LS gene possesses a complex, highly structured domain.
- These structural features likely play a significant role in the transcriptional regulation of RpII LS.
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