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Bacteria/blood/material interactions. I. Injected and preseeded slime-forming Staphylococcus epidermidis in flowing
M R Brunstedt1, S Sapatnekar, K R Rubin
1Department of Macromolecular Science, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Journal of Biomedical Materials Research
|April 1, 1995
Summary
This study investigated blood-material interactions with Staphylococcus epidermidis. Semicrystalline polymers showed the lowest bacterial adhesion and blood activation, suggesting better biocompatibility for cardiovascular materials.
Area of Science:
- Biomaterials Science
- Medical Microbiology
- Immunology
Background:
- Cardiovascular materials can interact with blood components and bacteria.
- Understanding these interactions is crucial for developing safer medical devices.
Purpose of the Study:
- To investigate blood-material interactions with slime-forming Staphylococcus epidermidis.
- To evaluate the influence of material surface properties on bacterial adhesion and blood activation.
Main Methods:
- In vitro recirculation model using human blood and Staphylococcus epidermidis.
- Testing various cardiovascular materials with preseeded or injected bacterial conditions.
- Measuring plasma levels of fibrinopeptide A (FpA) and C3a, and adhesion of white blood cells (WBCs) and platelets.
Main Results:
- Staphylococcus epidermidis adhered to nonsmooth materials, elevating FpA and C3a levels.
- Injected bacteria increased WBC and platelet adhesion and release of thrombospondin and platelet factor 4 (PF4).
- Semicrystalline polymers exhibited the lowest bacterial adhesion and blood activation compared to halogenated and amorphous substrates.
Conclusions:
- Material surface properties significantly influence bacterial adhesion and blood activation.
- Semicrystalline polymer substrates demonstrate superior biocompatibility in this in vitro model.
- Findings provide insights for designing cardiovascular materials with reduced thrombotic and inflammatory responses.