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Related Experiment Videos

Mutants lacking myosin II cannot resist forces generated during multicellular morphogenesis

E Shelden1, D A Knecht

  • 1Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269, USA.

Journal of Cell Science
|March 1, 1995
PubMed
Summary
This summary is machine-generated.

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Myosin II mutant cells in Dictyostelium discoideum show altered behavior and morphology during multicellular development, highlighting the protein's crucial role in cell movement and tissue formation.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biophysics

Background:

  • Multicellular development relies on coordinated cell movement and interaction.
  • Myosin II is a key motor protein involved in cellular processes.
  • Understanding cell behavior in aggregates is crucial for developmental studies.

Purpose of the Study:

  • To investigate the role of myosin II in cell behavior during early multicellular development.
  • To quantify the movement and morphology of wild-type and myosin II mutant cells in Dictyostelium discoideum.
  • To analyze the impact of myosin II on cell-cell interactions within developing aggregates.

Main Methods:

  • Fluorescent labeling and confocal microscopy for cell visualization.
  • Computer-assisted motion analysis to quantify cell behavior.

Related Experiment Videos

  • Observation of cells within aggregation streams and in isolation.

    • Main Results:

    • Myosin II mutant cells segregated to the edges of aggregation streams, unlike randomly distributed wild-type cells.
    • Mutant cells exhibited altered movement rates and failed to show periodic movement compared to wild-type cells.
    • Myosin II mutant cells displayed significant morphological deformations within wild-type streams, indicating a weakened cellular cortex.

    Conclusions:

    • Myosin II is essential for proper cell penetration and movement within multicellular aggregates.
    • The loss of myosin II severely impacts cell morphology and mechanical stability during development.
    • Cellular forces generated by wild-type cells can influence the behavior and morphology of neighboring mutant cells.
    • The study introduces advanced techniques for analyzing cell behavior in multicellular contexts.