Related Experiment Video
Updated: Jul 19, 2026
09:15
Electrochemiluminescence Assays for Human Islet Autoantibodies
Published on: March 23, 2018
Immunoassay for serum thyroxine monitored by chemiluminescence
Journal of Immunological Methods
|January 1, 1979
Summary
This study presents a novel chemiluminescence immunoassay for measuring thyroxine (T4) in serum. The method demonstrates good precision and accuracy, making it suitable for clinical diagnostics.
Area of Science:
- Clinical Chemistry
- Biochemical Assays
- Immunodiagnostics
Background:
- Thyroxine (T4) is a crucial thyroid hormone.
- Accurate T4 measurement is vital for diagnosing thyroid disorders.
- Existing immunoassays may have limitations in precision or interference.
Purpose of the Study:
- To evaluate a new chemiluminescence immunoassay (CLIA) for thyroxine (T4) determination.
- To assess the performance of the CLIA using clinical serum samples.
- To compare the CLIA results with a reference assay.
Main Methods:
- Sequential application of serum and thyroxine-label conjugate to Sephadex G-25 columns.
- Washing of unbound components with barbital buffer.
- Antibody-mediated binding and elution.
- Chemiluminescent detection of bound thyroxine-label conjugate.
- Quantification of T4 based on peak light intensity.
Main Results:
- The intra-assay precision was +/-5% coefficient of variation (C.V.).
- Statistical comparison with a reference assay showed acceptable agreement (y = 0.95x + 5.9, r = 0.98).
- The method demonstrated good correlation with established T4 measurement techniques.
Conclusions:
- The evaluated chemiluminescence immunoassay is a precise and accurate method for T4 quantification.
- This CLIA offers a reliable option for clinical laboratory diagnostics.
- The assay is suitable for routine measurement of thyroxine levels in patient serum.
Related Concept Videos
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
01:14Photoluminescence: Applications
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
01:20Synthesis and Regulation of Thyroid Hormones
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...