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Developmental changes in mRNA encoding cardiac Na+/H+ exchanger (NHE-1) in rabbit

F Chen1, J M Jarmakani, C Van Dop

  • 1Division of Pediatric Cardiology, University of California, Los Angeles 90095-1752, USA.

Biochemical and Biophysical Research Communications
|July 26, 1995
PubMed
Summary

Newborn rabbit hearts show faster recovery from acidosis due to higher Na+/H+ exchanger-1 (NHE-1) activity. NHE-1 mRNA levels are significantly higher in fetal and newborn hearts compared to adult hearts, explaining this difference.

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Area of Science:

  • Cardiovascular Physiology
  • Molecular Biology
  • Developmental Biology

Background:

  • Neonatal cardiac contractility recovers faster during acidosis than adult hearts.
  • This difference is linked to the activity of the Na+/H+ exchanger (NHE).

Purpose of the Study:

  • To investigate the developmental changes in cardiac Na+/H+ exchanger-1 (NHE-1) mRNA expression in rabbits.
  • To correlate NHE-1 mRNA levels with functional differences in cardiac contractility during acidosis.

Main Methods:

  • Quantification of NHE-1 mRNA in fetal, newborn, and adult rabbit ventricles.
  • Utilized reverse transcription-polymerase chain reaction (RT-PCR) and RNase protection assay.
  • GAPD mRNA was used as an internal standard for normalization.

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Main Results:

  • NHE-1 mRNA levels were similar in fetal and newborn rabbit hearts.
  • NHE-1 mRNA expression was significantly higher (1.7x and 1.6x) in fetal and newborn hearts compared to adult hearts.
  • These findings correlate with previously observed developmental changes in Na+/H+ exchanger activity.

Conclusions:

  • Cardiac NHE-1 mRNA expression is developmentally regulated in rabbits.
  • Higher NHE-1 mRNA levels in younger rabbits likely contribute to their enhanced ability to handle acidosis.
  • This study highlights the molecular basis for improved neonatal cardiac function under stress.