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Endothelial lined skeletal muscle ventricles: open and percutaneous seeding techniques
G A Thomas1, P I Lelkes, D M Chick
1Department of Surgery, Wayne State University School of Medicine, Detroit, Michigan, USA.
Journal of Cardiac Surgery
|May 1, 1995
Summary
Skeletal muscle ventricles (SMVs) can be successfully lined with endothelial cells using both open and percutaneous seeding methods. This research demonstrates a viable technique for creating enhanced vascularized tissues.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Vascular Biology
Background:
- Skeletal muscle ventricles (SMVs) are engineered tissues with potential for cardiovascular applications.
- Endothelialization is crucial for the function and integration of engineered tissues.
Purpose of the Study:
- To evaluate the efficacy of endothelial cell seeding in SMVs.
- To compare open versus percutaneous techniques for endothelial cell delivery.
Main Methods:
- Constructed twelve bilateral SMVs in six dogs using latissimus dorsi muscle and a 30 cc mandrel.
- Seeded SMVs with allogeneic cultured canine endothelial cells (8 x 10^6 cells/pouch) via open or percutaneous injection.
- Assessed endothelialization using fluorescent microscopy (KLH-2, dilacetylated LDL, von Willebrand factor) and electron microscopy.
Main Results:
- Viable, adherent endothelial cells were confirmed in all seeded SMVs.
- A continuous endothelial monolayer was observed, particularly at the apex.
- No significant differences in cell distribution or morphology were found between open and percutaneous seeding techniques.
Conclusions:
- Both open and percutaneous techniques are effective for seeding SMVs with an endothelial monolayer.
- These findings support the potential of SMVs for future vascularization strategies.