Related Experiment Videos
A single expression system for the display, purification and conjugation of single-chain antibodies
N Hayashi1, S Kipriyanov, P Fuchs
1Recombinant Antibody Group, German Cancer Research Center, Heidelberg.
Gene
|July 4, 1995
Abstract:
To facilitate the purification and conjugation of single-chain antibodies (scFv) selected from a phage display library, we have incorporated His6, an amber stop codon and a C-terminal Cys into a surface expression vector. The vector also contains a lacIq gene for improving the efficiency of regulation and a sequence coding for a marker peptide.