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Protein alterations in the skin of the mouse with radiation-induced gastroschisis
1Institut für Medizinische Strahlenbiologie, Universitätsklinikum Essen, Germany.
Abstract:
We have used two-dimensional gel electrophoresis to analyse proteins in the skin of mouse foetuses with and without gastroschisis after X-irradiation of embryos during the one-cell stage. An increased phosphorylation of two proteins and a shift in one glycoprotein were observed in the skin of irradiated foetuses with gastroschisis compared with the unirradiated normal controls. These protein changes are specifically associated with this malformation. We discuss the results in terms of mutations related to the irradiation of the one-cell embryo.
Insights
X-irradiation of mouse embryos can cause gastroschisis, a birth defect. Protein changes in the skin of affected fetuses suggest mutations linked to this developmental abnormality.
Area of Science:
- Developmental biology
- Toxicology
- Biochemistry
Background:
- Gastroschisis is a congenital malformation affecting the abdominal wall.
- X-irradiation of early-stage embryos can induce developmental abnormalities and mutations.
Purpose of the Study:
- To investigate protein alterations in mouse fetuses with gastroschisis induced by X-irradiation.
- To identify specific protein changes associated with X-ray-induced gastroschisis.
Main Methods:
- Two-dimensional gel electrophoresis was employed to analyze skin proteins.
- Mouse embryos at the one-cell stage were subjected to X-irradiation.
- Protein profiles of affected and control fetuses were compared.
Main Results:
- Increased phosphorylation of two proteins was observed in irradiated fetuses with gastroschisis.
- A shift in one glycoprotein was detected in the skin of affected fetuses.
- These protein alterations were specifically linked to the gastroschisis malformation.
Conclusions:
- The observed protein changes are indicative of mutations resulting from X-irradiation of one-cell embryos.
- These findings provide insights into the molecular mechanisms underlying radiation-induced gastroschisis.