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Hepatocyte growth factor induces gastric H+/K(+)-ATPase expression
L F Yee1, S H Carvajal, K J Andrews
1Department of Surgery, University of California, San Francisco 94143, USA.
The Journal of Surgical Research
|July 1, 1995
Summary
Hepatocyte growth factor (HGF) plays a crucial role in fetal gastric development. HGF infusion restored parietal cell function, indicated by H+/K(+)-ATPase expression, despite reduced gastric weight.
Area of Science:
- Gastroenterology
- Developmental Biology
- Cell Biology
Background:
- Fetal gastrointestinal tract development is poorly understood.
- Peptide growth factors are implicated in these developmental processes.
Purpose of the Study:
- To investigate the role of hepatocyte growth factor (HGF) in fetal gastric epithelium development.
- Specifically, to assess HGF's impact on parietal cell development.
Main Methods:
- Fetal rabbits underwent esophageal ligation (EL) to prevent amniotic fluid swallowing.
- Groups included controls, EL, EL with carrier, and EL with HGF infusion.
- Fetal stomachs were analyzed for weight, DNA, and H+/K(+)-ATPase expression at Day 28.
Main Results:
- Esophageal ligation (EL) significantly decreased gastric weight, DNA content, and H+/K(+)-ATPase expression.
- Intragastric carrier infusion did not reverse these effects.
- HGF infusion normalized H+/K(+)-ATPase expression, indicating restored parietal cell function, but did not significantly affect gastric weight or DNA content.
Conclusions:
- HGF is essential for maintaining normal H+/K(+)-ATPase expression in the fetal gastric epithelium.
- HGF plays a specific role in fetal parietal cell development and function.