Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thyroid stimulating hormone measurement using a third generation immunometric assay

C R Squire1, W D Fraser

  • 1University Department of Clinical Chemistry, Royal Liverpool University Hospital, UK.

Annals of Clinical Biochemistry
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Periconceptional and Gestational Vitamin D3 Restriction on Fetal Folliculogenesis and Anti-Mullerian Hormone Secretion Using Sheep as a Model.

Reproduction in domestic animals = Zuchthygiene·2024
Same author

Prevalence, incidence and determinants of QuantiFERON<sup>TM</sup> positivity in South African schoolchildren.

IJTLD open·2024
Same author

Sedentary behaviour and bone health in older adults: a systematic review.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2021
Same author

Hormonal contraceptive use, bone density and biochemical markers of bone metabolism in British Army recruits.

BMJ military health·2021
Same author

The effects of short-term low energy availability, achieved through diet or exercise, on cognitive function in oral contraceptive users and eumenorrheic women.

Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme·2021
Same author

Gestational hypercalcemia: Prevalence and biochemical profile.

The Journal of steroid biochemistry and molecular biology·2020
Same journal

Routine laboratory triage for NT-proBNP elevation may reflect cardiorenal frailty as much as heart failure specificity.

Annals of clinical biochemistry·2026
Same journal

From interpretable NT-proBNP prediction to equitable laboratory stewardship.

Annals of clinical biochemistry·2026
Same journal

Serum Sclerostin Levels in Children with Osteogenesis Imperfecta.

Annals of clinical biochemistry·2026
Same journal

Population-Specific HOMA-IR Reference Ranges in Iraq: Defining Insulin Sensitivity in a Middle Eastern Cohort.

Annals of clinical biochemistry·2026
Same journal

Optimizing Laboratory Quality with Sigma Metrics: Application of CLIA 2024 Total Allowable Error Guidelines.

Annals of clinical biochemistry·2026
Same journal

Suspected thermal amplitude of cryoglobulin likely more clinically significant than size detected: A case report.

Annals of clinical biochemistry·2026
See all related articles

Optimizing a third-generation thyroid stimulating hormone (TSH) assay significantly improved its sensitivity. This enhanced TSH assay provides better discrimination between thyroid states compared to older methods.

Area of Science:

  • Endocrinology
  • Clinical Chemistry
  • Assay Development

Background:

  • Thyroid stimulating hormone (TSH) assays are crucial for diagnosing thyroid disorders.
  • Second-generation TSH assays had limitations in sensitivity and discrimination.
  • Third-generation TSH assays aimed to improve diagnostic accuracy.

Purpose of the Study:

  • To optimize the performance of a third-generation TSH assay (Amerlite TSH-30).
  • To evaluate the sensitivity and clinical utility of the optimized assay.
  • To compare the optimized third-generation assay with a second-generation assay.

Main Methods:

  • Standard procedure evaluation for the Amerlite TSH-30 assay.
  • Modification of light emission measurement timing for standardization.

Related Experiment Videos

  • Comparison of the optimized assay with a second-generation DAKO TSH assay.

    • Main Results:

    • Optimized assay achieved significantly improved sensitivity (0.003 mU/L).
    • Excellent discrimination was observed between euthyroid, hypothyroid, and thyrotoxic subjects.
    • The third-generation assay showed a negative bias compared to the second-generation assay but offered better differentiation.

    Conclusions:

    • The optimized Amerlite TSH-30 assay demonstrates superior performance over second-generation assays.
    • Improved sensitivity and discrimination enhance the clinical utility of TSH testing.
    • This optimized assay aids in more accurate diagnosis and management of thyroid conditions.