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Two cDNA clones encoding 14-3-3 homologs from tomato fruit
B Laughner1, S D Lawrence, R J Ferl
1Department of Horticultural Sciences, University of Florida, Gainesville 32611, USA.
Biochimica Et Biophysica Acta
|July 25, 1995
Summary
Researchers identified two 14-3-3 protein-like genes in ripening tomatoes. These genes show distinct expression patterns, suggesting a role in tomato fruit development and regulation.
Area of Science:
- Plant Molecular Biology
- Biochemistry
- Genetics
Background:
- The 14-3-3 protein family acts as key regulatory molecules in eukaryotes.
- Understanding novel 14-3-3 homologs in plants can elucidate their roles in development.
Purpose of the Study:
- To identify and characterize novel 14-3-3 homologs in tomato fruit.
- To investigate the expression patterns of these genes during fruit ripening.
Main Methods:
- Immunoscreening of a ripening tomato fruit cDNA library.
- Nucleotide and amino acid sequence analysis of identified clones.
- Southern hybridization to assess gene copy number.
- Analysis of transcript accumulation patterns during fruit ripening.
Main Results:
- Two distinct tomato cDNA clones homologous to 14-3-3 proteins were isolated.
- These clones exhibit high nucleotide (71%) and amino acid (84%) identity.
- Sequence analysis revealed substitutions at the carboxy-terminus.
- Southern hybridization confirmed each clone represents a unique gene.
- Distinct transcript accumulation patterns were observed during fruit ripening.
Conclusions:
- The identified tomato 14-3-3 homologs are encoded by unique genes.
- Their differential expression during ripening suggests involvement in fruit development.
- These findings contribute to understanding plant protein regulation and fruit maturation.