Perfusion imaging of skin island flap blood flow by a scanning laser-Doppler technique

Conventional laser-Doppler perfusion measurements can only obtain information from a single site. Since superficial blood flow is heterogeneous, this is a serious limitation, particularly in studies of methods to improve skin flap survival. A scanning laser-Doppler instrument has been developed which provides both an image and quantitative information about perfusion of the superficial tissue. We have evaluated this instrument in a circumflex iliac artery island flap model in the pig. The validity of the model was demonstrated by fluorescein dye injection and histology. Elevation of flaps was found to increase proximal flap blood flow but to decrease it in the distal portion. In flaps subjected to arterial ischaemia (9 h) and reperfusion we found an early increase in proximal flow, which gradually extended distally over the first 40 to 60 min. 16 h later, flow had declined, compatible with reperfusion damage to the vasculature. We suggest that perfusion imaging may be a valuable technique for investigating the mechanisms and extent of reperfusion injury.