Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tumor necrosis factor-induced necrosis: a monocyte-mediated hypercoagulable effect

C R Spillert1, S Sun, R Ponnudurai

  • 1Department of Surgery, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103-2757, USA.

Journal of the National Medical Association
|July 1, 1995
PubMed
Summary

Tumor necrosis factor (TNF) can cause tissue necrosis, but requires incubation. TNF alone does not activate blood clotting; monocyte activation is necessary for TNF to induce hypercoagulability and thrombosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of methylprednisolone on coagulation.

Journal of the National Medical Association·2003
Same author

Nasal toxicity of cocaine: a hypercoagulable effect?

Journal of the National Medical Association·2000
Same author

Determination of hypercoagulable state in acute bronchospasm.

The Journal of the American Osteopathic Association·1999
Same author

Complete cartilaginous duplication of the rib cage found in a cadaver: a first reported case.

The American surgeon·1996
Same author

Cost-effectiveness of incorporating inactivated poliovirus vaccine into the routine childhood immunization schedule.

JAMA·1996
Same author

Hydrolysis of cyclic guanosine monophosphate and cyclic adenosine monophosphate by the penis and aorta of the diabetic rat.

British journal of urology·1996

Area of Science:

  • Immunology
  • Hematology
  • Oncology

Background:

  • The precise mechanisms underlying tumor necrosis factor's (TNF) necrotic effects remain unclear.
  • A proposed pathway involves TNF-induced monocyte activation, leading to tissue factor production and subsequent hypercoagulability.
  • This hypercoagulable state may result in thrombotic vascular occlusion and tissue necrosis.

Purpose of the Study:

  • To investigate the role of TNF in inducing procoagulant activity and tissue necrosis.
  • To determine if TNF alone, or in conjunction with monocyte activation, causes hypercoagulability.

Main Methods:

  • Modified recalcification time assays were performed on blood samples.
  • Samples were treated with albumin (control), endotoxin-containing saline, or albumin with TNF (450 units).

Related Experiment Videos

  • The effect of incubation time on procoagulant activity was assessed.

    • Main Results:

    • No significant differences in recalcification times were observed between the groups without incubation.
    • Incubation was required for TNF to exhibit any effect on the blood samples.
    • TNF alone, without prior monocyte activation, did not demonstrate procoagulant activity.

    Conclusions:

    • Tumor necrosis factor (TNF) can induce tissue necrosis, but this effect is dependent on incubation.
    • TNF itself lacks direct procoagulant activity.
    • Monocyte activation is a critical prerequisite for TNF to initiate a procoagulant cascade leading to thrombosis and necrosis.