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Updated: Aug 11, 2026

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
Published on: November 20, 2013
Quantitative biological electron probe microanalysis with a wavelength dispersive spectrometer
A Pogorelov1, V Pogorelova, N V Repin
1Institute of Theoretical and Experimental Biophysics of the Russian Academy, Moscow Province.
Abstract:
This paper describes the details of quantitative electron probe microanalysis (EPMA) performed with a wavelength dispersive spectrometer (WDS). EPMA was carried out on the giant neuron of a fresh frozen ganglion from the snail Lymnaea stagnalis. The freeze-dried cryosections were compared with sections of freeze-dried, embedded tissue. It was found, that in the ganglion there are two kinds of neurons with a different chlorine concentration of 11 mmole/liter and 32 mmole/liter. Isolated neurons in culture were shown to differ in elemental composition from those in the ganglion tissue.
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