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Use of in vitro systems for X-ray microanalysis
J Hongpaisan1, A C Mörk, G M Roomans
1Department of Human Anatomy, University of Uppsala, Sweden.
Scanning Microscopy. Supplement
|January 1, 1994
Summary
Cryopreservation challenges in pathology are overcome using in vitro systems. Cell cultures and tissue samples in physiological buffers show significant ion shifts, crucial for X-ray microanalysis.
Area of Science:
- Pathology
- Biophysics
- Cell Biology
Background:
- X-ray microanalysis in human pathology often requires cryopreserved tissue.
- Optimal tissue freezing and in vivo experiments present significant challenges.
- In vitro systems offer a viable alternative for tissue analysis and substance testing.
Purpose of the Study:
- To evaluate the utility of in vitro systems and cell cultures for X-ray microanalysis.
- To investigate ion concentration changes in animal tissues during incubation.
- To assess the response of in vitro systems to pharmacological stimulation.
Main Methods:
- Incubation of animal tissues (brain, liver, pancreas, submandibular gland) in physiological buffer.
- X-ray microanalysis of cryoprepared tissue and cell cultures.
- Stimulation of submandibular gland in vitro and analysis of immortalized sweat gland cells.
Main Results:
- Incubation in physiological buffer altered Na, K, and Cl concentrations in animal tissues.
- Prolonged incubation led to further ion shifts in brain and liver tissues.
- In vitro submandibular gland responded similarly to in situ stimulation; sweat gland cells lost Cl upon cAMP stimulation.
Conclusions:
- In vitro systems and cell cultures are valuable for X-ray microanalysis in pathology.
- Physiological buffer incubation induces significant, predictable ion changes in tissues.
- Cellular responses to stimuli in vitro are consistent with in vivo observations, validating their use.