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Standardless analysis of biological tissue sections

W A Nicholson1

  • 1Department of Physics and Astronomy, University of Glasgow, UK.

Scanning Microscopy. Supplement
|January 1, 1994
PubMed
Summary

This study introduces a formula-based method for standardless X-ray microanalysis of biological samples. It calculates elemental concentrations by evaluating X-ray production cross-sections, improving accuracy in biological sample analysis.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biophysics

Background:

  • X-ray microanalysis of thin biological samples typically requires standards for elemental concentration determination.
  • Existing methods rely on comparing sample spectra to those of known standards.

Purpose of the Study:

  • To present a formula-based method for standardless X-ray microanalysis of thin biological samples.
  • To evaluate the physical processes of characteristic and bremsstrahlung X-ray production for quantitation.
  • To describe the incorporation of these calculations into standardless quantitation techniques.

Main Methods:

  • Utilizing formulae to evaluate factors for converting X-ray data into elemental concentrations.
  • Calculating X-ray production cross-sections for characteristic and bremsstrahlung radiation.
  • Applying these calculations to standardless quantitation methods for biological specimens.

Main Results:

  • Demonstrated a method for standardless quantitation by calculating X-ray production cross-sections.
  • Presented calculations for characteristic and bremsstrahlung cross-sections under typical analytical conditions.
  • Provided a framework for accurate elemental analysis of biological samples without physical standards.

Conclusions:

  • Formula-based evaluation of X-ray production cross-sections enables standardless quantitation for biological samples.
  • This approach simplifies elemental analysis by removing the need for element-specific standards.
  • The method offers a viable alternative for elemental concentration determination in thin biological specimens.

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