Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ion microscopy in biology

G H Morrison1, I Gay, S Chandra

  • 1Baker Laboratory of Chemistry, Cornell University, Ithaca, NY 14853, USA.

Scanning Microscopy. Supplement
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differentiation of human dental stem cells reveals a role for microRNA-218.

Journal of periodontal research·2013
Same author

Parameter control, characterization, and optimization in the fabrication of optical fiber near-field probes.

Applied optics·2010
Same author

Postoperative Reactions.

Canadian Medical Association journal·2010
Same author

The Sault Ste. Marie Health Centre.

Canadian Medical Association journal·2010
Same author

Runx2, osx, and dspp in tooth development.

Journal of dental research·2009
Same author

Development of an integrated intraspecific map of chickpea (Cicer arietinum L.) using two recombinant inbred line populations.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2007
Same journal

Proceedings of the 14th Pfefferkorn Conference. Belleville, Illinois, USA. August 6-11, 1995.

Scanning microscopy. Supplement·1996
Same journal

Advanced instrumentation and methodology related to cryoultramicrotomy: a review.

Scanning microscopy. Supplement·1996
Same journal

Aspects of cryofixation and cryosectioning for the observation of bulk biological samples in the hydrated state by cryoelectron microscopy.

Scanning microscopy. Supplement·1996
Same journal

In vitro systems and cultured cells as specimens for X-ray microanalysis.

Scanning microscopy. Supplement·1996
Same journal

X-ray microscopy: preparations for studies of frozen hydrated specimens.

Scanning microscopy. Supplement·1996
Same journal

Specimen preparation of the human cerebellar cortex for scanning electron microscopy using a t-butyl alcohol freeze-drying device.

Scanning microscopy. Supplement·1996
See all related articles

Ion microscopy, a sensitive mass spectrometry technique, visualizes element distribution in cells. This method aids in understanding ion transport and screening drugs for cancer therapy.

Area of Science:

  • Biophysics
  • Cell Biology
  • Analytical Chemistry

Background:

  • Ion microscopy is a mass spectrometry-based isotopic imaging technique.
  • It offers high sensitivity for imaging major and minor elements at subcellular resolutions.
  • Cryogenic sample preparation is crucial for ion transport studies.

Purpose of the Study:

  • To highlight the capabilities of ion microscopy in biological systems.
  • To demonstrate its application in studying ion transport and element distribution.
  • To showcase its utility in Boron Neutron Capture Therapy (BNCT) drug screening.

Main Methods:

  • Utilizing ion microscopy for real-time imaging of major elements (K, Na, Cl).
  • Employing stable isotopes as tracers for imaging endogenous and transported elements.

Related Experiment Videos

  • Performing correlative imaging with laser scanning confocal microscopy.

    • Main Results:

    • Identified the Golgi apparatus as a calcium-storing organelle through correlative imaging.
    • Demonstrated the capability to image low-concentration elements like calcium with integration time.
    • Showcased ion microscopy's potential for rapid screening of boronated drugs for BNCT.

    Conclusions:

    • Ion microscopy provides unprecedented insights into ion transport and element distribution in biological systems.
    • It is a valuable tool for identifying subcellular compartments and their functions, such as calcium storage.
    • The technique is crucial for advancing the understanding and application of BNCT for cancer treatment.