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GA3-regulated cDNAs from Hordeum vulgare leaves
1Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Plant Molecular Biology
|August 1, 1995
Summary
Gibberellic acid (GA3) application reverses the extreme dwarf phenotype in barley mutant dbg 576, inducing partial fertility. This treatment upregulates specific genes, ES1A and ES2A, in leaf blades, suggesting their role in growth regulation.
Area of Science:
- Plant Molecular Biology
- Plant Physiology
- Genetics
Background:
- The barley mutant dbg 576 exhibits a severe vegetative dwarf phenotype.
- Application of gibberellic acid (GA3) effectively reverses this dwarfism and partially restores fertility in mutant florets.
Purpose of the Study:
- To identify genes involved in the GA3-mediated growth response in the barley mutant dbg 576.
- To characterize the expression patterns and potential functions of these responsive genes.
Main Methods:
- Differential screening of a cDNA library from GA3-treated mutant leaf blades using subtracted probes.
- Isolation and characterization of cDNA clones ES1A and ES2A.
- Analysis of mRNA expression levels and protein homology.
Main Results:
- Two cDNA clones, ES1A and ES2A, were identified, showing rapid and transient increases in mRNA levels post-GA3 treatment.
- ES1A and ES2A mRNA accumulation is specific to leaf blades.
- ES1A encodes a protein homologous to mammalian epidermal growth factors (EGFs).
- ES2A encodes a protein homologous to the D7 family of late embryogenesis-abundant (LEA) proteins.
Conclusions:
- The barley genes ES1A and ES2A are rapidly induced by GA3 in a leaf-specific manner, playing a role in growth regulation.
- The identified proteins suggest novel functions for EGF-like and LEA-like proteins in plant vegetative growth and development.