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Sequence-specific DNA binding activities of nuclear matrix proteins of mammalian lens epithelial cells
M Bagchi1, A Van Wijnen, M Katar
1Department of Anatomy and Cell Biology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA.
Abstract:
This study examines matrix and nonmatrix nuclear proteins of the rabbit lens epithelial cells. The nuclear matrix proteins were isolated by modified Penman technique, which requires presence of detergents and nucleases, whereas nonmatrix nuclear proteins were obtained by high salt extraction. The data from these experiments revealed presence of DNA binding activities for SP-1 and OCT-1 proteins in both matrix and non-matrix compartments of rabbit lens epithelial cells. Comparison of the relative abundance of SP-1 and OCT-1 binding activities in nuclear matrix and nonmatrix fractions suggest the distribution between these two compartments is cell type specific and possibly related to the control of cell growth.