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Related Experiment Videos

ATL cells recognize self class II HLA antigens: implication to leukemogenesis

M Matsuoka1, T Hattori, Y Nishimura

  • 1Second Department of Internal Medicine, Kumamoto University School of Medicine, Japan.

Leukemia
|August 1, 1995
PubMed
Summary

Adult T cell leukemia (ATL) cells may be driven by self-antigen stimulation. This study investigated if ATL cells respond to self-antigens, suggesting a role for T cell receptor (TCR)/CD3 complex signaling in leukemogenesis.

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Area of Science:

  • Immunology
  • Oncology
  • Cell Biology

Background:

  • Adult T cell leukemia (ATL) cells exhibit reduced T cell receptor (TCR)/CD3 complex expression in vivo.
  • Antigenic stimulation is known to modulate TCR/CD3 complex expression on T lymphocytes.

Purpose of the Study:

  • To investigate the hypothesis that antigenic stimulation down-regulates the antigen receptor of ATL cells, potentially contributing to leukemogenesis.
  • To determine if fresh ATL cells respond to autologous and allogeneic lymphoid cell lines.

Main Methods:

  • Culturing fresh ATL cells from three patients with autologous and allogeneic lymphoid cell lines.
  • Utilizing monoclonal antibodies (anti-CD3, anti-HLA-DQ, anti-HLA-DR) to inhibit proliferation and identify specific antigen recognition.

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Main Results:

  • ATL cells from two out of three cases proliferated in response to autologous cell lines.
  • In one case, proliferation was inhibited by anti-CD3 and anti-HLA-DQ antibodies, indicating recognition of self-HLA-DQ.
  • In another case, proliferation was suppressed by anti-CD3 and HLA-DR antibodies.

Conclusions:

  • ATL cells in some cases originate from autoreactive T lymphocytes.
  • TCR/CD3 complex stimulation by self-antigens likely plays a significant role in the in vivo leukemogenesis of ATL.