Expression of a dopamine transporter in PC12h cells: an immunohistochemical study with an antipeptide antibody

Two kinds of oligopeptides, based on the amino acid sequences of No. 217-232 and 374-387 of a rat dopamine transporter, were designed and chemically synthesized. Five clones of the monoclonal antibodies against these peptides were produced with the in vitro immunization method. Two of them have recognized a protein of M(r) approximately 85,000 in a synaptosomal fraction of the rat striatum. It is probable that the protein M(r) approximately 85,000 corresponds to a dopamine transporter in the nerve terminal of the dopamine neurons in the striatum. The expression of dopamine transporter was immunohistochemically examined with these antibodies in PC12h cells. The immunoreactivity was detected on the surface membrane of the cells.