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Age-related alterations in cell division and cell cycle kinetics in control and trimethyltin-treated lymphocytes of
1Biochemistry Unit, Indian Statistical Institute, Calcutta.
Abstract:
Trimethyltin chloride induced age-related suppression of cell division and cell cycle kinetics in human peripheral blood lymphocytes cultured in RPMI 1640 culture medium supplemented with human AB serum, phytohemagglutinin and bromodeoxyuridine. A high frequency of M1 (first metaphase) cells was seen in cultures treated with a high dose (C1 = 1.0 microgram per culture) and in lymphocytes from donors in the age range 40-70 years. The delay in cell division and cell cycle kinetics may indicate a longer duration in DNA synthesis induced by trimethyltin chloride in aged lymphocytes.
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