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Energetics of Cd2+ efflux system in cadmium-resistant Staphylococcus aureus 17810R
Acta Biochimica Polonica
|January 1, 1995
Abstract:
Energetics of 109Cd efflux in resting cells of cadmium-resistant Staphylococcus aureus 17810R was assayed in 1 or 100 mM potassium/sodium phosphate buffer, pH7 (PB). Experiments with the use of inhibitors and ionophores showed that Cd2+ extrusion in this organism required ATP and either a pH gradient (delta pH) in 1 mM PB or membrane potential (delta psi) in 100 mM PB. The role of high phosphate ion concentration in delta psi-dependent Cd2+ efflux is discussed.