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Fluorescent microspheres to measure organ perfusion: validation of a simplified sample processing technique
M F Van Oosterhout1, H M Willigers, R S Reneman
1Department of Physiology, University of Limburg, Maastricht, The Netherlands.
The American Journal of Physiology
|August 1, 1995
Summary
A simplified fluorescent microsphere (FM) processing method offers reliable blood flow measurement. This technique provides high recovery and good correlation with radioactivity labeled microspheres (RM) for organ and myocardial blood flow analysis.
Area of Science:
- Physiology
- Biomedical Engineering
- Cardiovascular Research
Background:
- Nonradioactive microsphere techniques for blood flow measurement are often time-consuming and complex.
- A simplified processing method for fluorescent microspheres (FM) is needed to improve efficiency and accessibility.
Purpose of the Study:
- To develop and validate a simplified processing method for fluorescent microsphere (FM) techniques.
- To compare the accuracy of the simplified FM method with traditional radioactivity labeled microspheres (RM) for measuring blood flow.
Main Methods:
- Developed a simplified processing protocol for FM, involving sample digestion, centrifugation, and fluorescence measurement.
- Injected multiple FM and RM labels simultaneously in dogs with coronary artery stenosis for inter- and intramethod comparisons.
- Analyzed blood, myocardium, and other organ samples to assess microsphere recovery and blood flow distribution.
Main Results:
- The simplified FM processing method achieved approximately 100% microsphere recovery.
- High correlations were observed between FM and RM methods for inter- and intramethod comparisons (r = 0.985 +/- 0.01).
- Endocardial-to-epicardial blood flow ratios measured by FM and RM showed strong correlation (r = 0.974).
Conclusions:
- The simplified FM processing method provides a reliable and efficient means for measuring blood flow in various organs, including ischemic myocardium.
- This technique offers a viable alternative to traditional methods, with high accuracy and recovery rates.
- Careful selection of FM labels is recommended, as the blue microsphere label showed a lower intermethod correlation.