[A preliminary study on metastasis-associated gene screened by monoclonal antibodies HIL]

The cDNA expression libraries derived from a highly metastatic cell subline Anip[973] and from the poorly metastatic, parental cell line, AGZY-83a were screened by monoclonal antibodies HIL. A positive clone (H4-D) from the Anip[973] cDNA library was isolated and its nucleotide sequence was determined. This clone contained 978 bp with an open reading frame of 318 bp encoding a polypeptide consisting of 106 amino acids. The H4-D cDNA sequence showed 85% homology with a human propionyl-CoA carboxylase alpha-chain. In western blotting analysis, the MoAb H4 recognized 2 bands (15KD and 27KD) of Anip[973] cell membrane proteins. The mRNA expression of H4-D was higher in Anip[973] cells than in AGZY-83a cells. The metastatic potential of Anip[973] cells was markedly decreased after being pretreated with MoAb H4. The above findings indicate that H4-D has a certain relationship with the metastatic phenotype of Anip[973] cells.